INTRODUCTION OF EXOGENOUS WILD SOYBEAN DNA INTO CULTIVATED SOYBEAN AND RAPD MOLECULAR VERIFICATION

The exogenous total DNA of the wild high-protein soybean was transferr ed to cultivated soybean through the pollen tube channel and the genom ic variation of the transformed progeny was detected by the method of RAPD (Random Amplified Polymorphic DNA). Distinguished variations were found in one of the 7 transformed plants of the first generation (D-1 ), of which the traits of fruition, outward appearance, leaf shape and ;newer colour were almost identical with those of the recipient parent ; of which grain weight, seed coat colour and stem strength were situa ted between the two parents; and there were greatly more pods per plan t and 12.5% higher content of protein in seeds than that of the recipi ent parent. All the properties have been invariably inherited for 3 ge nerations. Through RAPD analysis of the genomes of the donor, the reci pient and the transformed progeny (D;) as a group, DNA polymorphisms w ere found in amplified products by 24 of 150 primers. The results prov e that the exogenous DNA caused the distinct variance of the genome. T he authors infer that the homogeneous recombination of large exogenous DNA is the main cause for the variance.