S. Shiotani et al., CELL RECOGNITION BY IMAGE-PROCESSING - RECOGNITION OF DEAD OR LIVING PLANT-CELLS BY NEURAL-NETWORK, JSME international journal. Series C, dynamics, control, robotics, design and manufacturing, 37(1), 1994, pp. 202-208
This paper deals with determination of whether protoplasts are alive o
r not. The protoplasts are plant cells which are removed cell walls, a
nd have been used for cell fusion to generate new species of plants. C
ell fusion uses living protoplasts, and therefore, a method of life or
death determination for protoplasts is necessary. FDA (fluorescein di
acetate) has been used for this purpose. However, FDA has not been app
lied for cell fusion because it is toxic to protoplasts. In this paper
, we propose a new harmless method for the above determination using t
he image processing technique and an artificial neural network. The pr
oposed method measures shape and colors of protoplasts using image pro
cessing for sensory information. The neural network determines whether
protoplasts are living or not based on the sensory information. Exper
iments were performed in order to show the effectiveness of the propos
ed method. As the result, the proposed method is found to be able to r
ecognize states of protoplasts with an accuracy of 85 percent. This me
thod is promising for determining life or death of protoplasts in cell
fusion, instead of FDA.