FUSARIUM-GRAMINEARUM-A-3 5 AS A NOVEL HOST FOR HETEROLOGOUS PROTEIN-PRODUCTION/

We describe a novel fungal expression system which utilizes the Quorn( R) myco-protein fungus Fusarium graminearum A 3/5. A transformation sy stem was developed for F. graminearum and was used to introduce the co ding and regulatory regions of trypsin gene from Fusarium oxysporum. T he protein was efficiently expressed, processed and secreted by the re combinant host strain. In addition, the promoter and terminator of the F. oxysporum trypsin gene have been successfully utilized to drive th e expression of a cellulase gene from Scytalidium thermophilum and a l ipase gene from Thermomyces lanuginosus in F. graminearum.