STRUCTURE AND LIGAND RECOGNITION OF THE PHOSPHOTYROSINE BINDING DOMAIN OF SHC

The nuclear magnetic resonance structure of the phosphotyrosine bindin g (PTB) domain of Shc complexed to a phosphopeptide reveals an alterna tive means of recognizing tryosine-phosphorylated proteins. Unlike in SH2 domains, the phosphopeptide forms an antiparallel beta-strand with a beta-sheet of the protein, interacts with a hydrophobic pocket thro ugh the (pY-5) residue, and adopts a beta-turn. The PTB domain is stru cturally similar to pleckstrin homology domains (a alpha-sandwich capp ed by an alpha-helix) and binds to acidic phospholipids, suggesting a possible role in membrane localization.