EVALUATION OF SENSITIVITY OF FLOW-CYTOMETRY IN DETECTING ANEUPLOIDY IN WHEAT USING DISOMIC AND DITELOSOMIC WHEAT-RYE ADDITION LINES

Flow cytometric DNA. analysis was used to study changes in nuclear DNA content induced by the addition of complete or telosomic rye chromoso mes into the genome of common wheat (Triticum aestivum L.). The DNA co ntent of each addition Line was determined by comparison with an inter nal reference value and was expressed as a difference with respect to the original wheat parental line. A 1.84% difference in the DNA conten t could be detected, Nuclei were now sorted and the presence of rye ch romatin in the nuclei with the higher DNA content was demonstrated by Southern hybridization, Flow cytometry was proven to be sensitive enou gh to detect the small DNA content deviations that are expected to occ ur in aneuploid plants of wheat. (C) 1995 Wiley-Liss, Inc.