Allele and genotype frequencies for 7 tetrameric short tandem repeat l
oci were determined in a Spanish population sample (N = 186-244) using
PCR and subsequent analysis of the PCR products by denaturing polyacr
ylamide gel electrophoresis followed by silver staining. The loci were
HUTMFES/FPS, HUMVWA, HUMTHO1, HUMF13B, HUMCSF1PO, HtSMF13A1 and HUMT-
POX and all loci met Hardy-Weinberg expectations, In addition, little
evidence was found for association of alleles among the 7 loci. Thus t
he allele frequency data can be used in identity testing to estimate t
he frequency of a multiple PCR-based DNA profile in the Spanish popula
tion.