The glucose and lactose effect on the steroid biotransformation reacti
ons by Mycobacterium ap. NRRL,B-3683 was studied The reduction reactio
ns increased at pH 7 and when sugars are added The oxidation reactions
are highers at pH 8 without any sugar added The dehydrogenation react
ion is inhibited when an endogenous carbon source is present in the me
dia.