G. Curutchet et al., INDIRECT BIOLEACHING OF COVELLITE BY THIOBACILLUS THIOOXIDANS WITH ANOXIDANT AGENT, Biotechnology letters, 17(11), 1995, pp. 1251-1256
High copper extraction by the action of Thiobacillus thiooxidans (T.t.
) on covellite in presence of iron(III) is explained by indirect mecha
nism, in which T.t. only oxidizes the layer of sulphur that covers the
sulphide surface allowing sulphide oxidation by iron(III). In culture
s on elemental sulphur with iron(III) T.t. is not able to use iron(III
) as an acceptor of electrons in sulphur oxidation; iron(III) only oxi
dizes those intermediates which were generated in the aerobic oxidatio
n of sulphur.