C. Pedersen et I. Lindelaursen, THE RELATIONSHIP BETWEEN PHYSICAL AND GENETIC DISTANCES AT THE HOR1 AND HOR2 LOCI OF BARLEY ESTIMATED BY 2-COLOR FLUORESCENT IN-SITU HYBRIDIZATION, Theoretical and Applied Genetics, 91(6-7), 1995, pp. 941-946
The hordeins are the major class of storage proteins in barley. They a
re encoded by multigene families. The B- and C-hordein loci have been
mapped physically to the distal end of chromosome 5 (1I) of cultivated
barley by fluorescent in situ hybridization. Based on measurements of
chromosomal distances between the two hordein loci, the relationship
between genetic and physical distances has been estimated to be about
1 mega base pairs per centiMorgan. This is four times higher than the
mean value for the barley genome as a whole and confirms the tendency
to increased recombination in distal chromosome regions. The resolving
power of two-colour FISH is discussed. It is concluded that the metho
d is suitable for estimating the relationship between genetic and phys
ical distances of regions of about 10 Mbp or larger.