Y. Morimitsu et al., PROTEIN GLYCATION INHIBITORS FROM THYME (THYMUS-VULGARIS), Bioscience, biotechnology, and biochemistry, 59(11), 1995, pp. 2018-2021
Nonenzymatic glycation of bovine serum albumin (BSA) was inhibited in
vitro by some extracts of 34 kinds of spices, The methanol extract of
thyme (Thymus vulgaris) had the most potent inhibitory activity among
them. Chromatographic purification yielded four flavonoids, quercetin
(1), eriodictyol (2), 5,6,4'-trihydroxy-7,8,3'-trimethoxyflavone (3),
and cirsilineol (4). These known flavonoids suppressed the levels of a
dvanced glycation end products (AGEs) and fructosamines, shown by the
measurement of specific fluorescent groups and the reduction of nitrob
lue tetrazolium (NET), respectively, The inhibitory activities were co
mpared with those of other structure-related flavonoids and aminoguani
dine.