ENZYMATIC SYNTHESES OF 2 STABLE (-)-EPIGALLOCATECHIN GALLATE-GLUCOSIDES BY SUCROSE PHOSPHORYLASE

Authors
KITAO S MATSUDO T SAITOH M HORIUCHI T SEKINE H
Citation
S. Kitao et al., ENZYMATIC SYNTHESES OF 2 STABLE (-)-EPIGALLOCATECHIN GALLATE-GLUCOSIDES BY SUCROSE PHOSPHORYLASE, Bioscience, biotechnology, and biochemistry, 59(11), 1995, pp. 2167-2169
Citations number
8
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
59
Issue
11
Year of publication
1995
Pages
2167 - 2169
Database
ISI
SICI code
0916-8451(1995)59:11<2167:ESO2S(>2.0.ZU;2-4
Abstract
Sucrose phosphorylase from Leuconostoc mesenteroides catalyzed transgl ucosylation from sucrose to (-)-epigallocatechin gallate. Two main tra nsfer products were obtained, and their structures were identified as( -)-epigallocatechin gallate 4'-O-alpha-D-glucopyranoside and (-)-epiga llocatechin gallate 4',4''-O-alpha-D-diglucopyranoside on the basis of secondary ion mass spectrometry analysis, component analyses of their enzymatic hydrolyzates, and nuclear magnetic resonance analysis, The stability of (-)-epigallocatechin gallate was greatly increased by the glucosylation.