Occurrence of free D-alanine was verified in various marine macroalgae
by reversed-phase HPLC analysis, The presence of free D-alanine was d
etected in 15 species (63%) out of the 23 species of algae examined. T
he free D-alanine content was high in Hizikia fusiformis, Heterochorda
ria abietina, and Sargassum nigrifolium of Phaeophyta (96, 83, and 78
nmol/g wet weight, respectively), The D-alanine contents in various pa
rts of H. fusiformis were measured and found to be highest in the blad
e followed by the main branch, and was lowest in the adhesive root.