Hw. Lee et al., CHARACTERIZATION OF KINETIC-PARAMETERS AND METABOLIC TRANSITION OF CORYNEBACTERIUM-GLUTAMICUM ON L-LYSINE PRODUCTION IN CONTINUOUS-CULTURE, Applied microbiology and biotechnology, 43(6), 1995, pp. 1019-1027
Kinetic parameters and physiological states of Corynebacterium glutami
cum at the growing and L-lysine-overproducing phase were characterised
in continuous culture on threonine-limited complex and minimal media.
High L-lysine productivity occurred at dilution rates ranging from 0.
1h(-1) to 0.3h(-1) on threonine-limited complex medium, and at dilutio
n rates ranging from 0.1h(-1) to 0.15h(-1) in minimal medium. L-Lysine
yields of 0.25 g/g (0.31 g/g as L-lysine hydrochloride) in complex me
dium, and of 0.17 g/g (0.21 g/g as L-lysine hydrochloride) in minimal
medium, corresponding respectively to intrinsic yields of 0.533 g/g an
d 0.572 g/g were obtained. These intrinsic yield factors are dosed to
the theoretical ones (0.608 g/g, 0.75 mol/mol). Intrinsic biomass yiel
ds were calculated as 0.658 g/g in complex medium and 0.283 g/g in min
imal medium. CO2 production has been clearly related to L-lysine produ
ction. According to our results on specific uptake rates and specific
productivities in complex medium, metabolic rearrangement should occur
during the transition from the growing phase to the L-lysine-overprod
ucing phase. This phenomenon was further investigated.