Bj. Dave et al., SHARED CYTOGENETIC ABNORMALITIES IN LUNG-TUMORS AND CORRESPONDING PERIPHERAL-BLOOD LYMPHOCYTES, International journal of oncology, 7(6), 1995, pp. 1297-1305
We analyzed chromosomal alterations in primary lung tumours and periph
eral blood lymphocytes (PBLs) from 10 lung cancer patients (nine with
non-small cell lung carcinoma and one with small cell lung carcinoma)
to determine whether there were shared chromosomal changes in the norm
al and diseased tissue of these cases. This study revealed that each p
aired sample had at least three chromosomes and two chromosomal region
s in common for structural rearrangements. The chromosomes most freque
ntly found structurally altered in paired analysis were 1 and 3 (60% e
ach), 5 (50%), 6, 7, and 9 (40% each), and 14 (30%). Chromosome region
3p13-3p21 was structurally rearranged in both the normal and tumour t
issues of three patients. Chromosome 3 was structurally rearranged in
all ten tumours. The chromosome arms most commonly affected in the tum
ours were 3p (nine times), 9p and 5q (eight times each), Ip and 7q (si
x times each), 10q and 11q (five times each), 14q and 6q (four times e
ach). The most frequently affected chromosomal regions in these tumour
s were (in decreasing order) 9p23-p24, 3p21-3p13, 5q11, 1p34, 7q22, an
d 11q13. Frequent polysomy of 7 and 12 and loss of D-group chromosomes
were also observed in the tumours analyzed. Comparing the changes fou
nd only in tumours with those found in both PBLs and tumours was helpf
ul in shedding some light on the probable sequence of genetic events l
eading to lung cancer. This investigation also offered compelling evid
ence that genomic instability at the chromosomal level in PBLs corresp
onds with the genetic changes observed in tumours indicating that PBL
analysis can help identify the early chromosomal changes in lung cance
r. PBL chromosomal analysis thus has a promising future in the genetic
analysis of lung cancers.