DIFFERENT SUBNUCLEAR LOCALIZATION OF WILD-TYPE AND MUTANT P53 IN HUMAN PROSTATE-CANCER CELLS

The growth suppressor protein p53 is abnormally expressed in a variety of different human tumor cells. We have analyzed the expression of p5 3 in cell cultures derived from tissues of radical prostatectomies and in the permanent prostate carcinoma cell line PC-3 using two differen t p53 specific monoclonal antibodies. With the wild-type specific mono clonal antibody PAb1620 we found p53 localized in nucleoli whereas onl y a few cells were positively stained in the nucleus with the mutant s pecific monoclonal antibody PAb240. Control experiments with p53 from SV80 cells which express wild-type p53 and HT29 cells expressing mutan t p53 documented the specificity of the monoclonal antibodies. The spe cificity of the antibodies in recognizing indeed p53 was demonstrated further by immunoprecipitation analysis of p53 from the same cell cult ures. Since p53 is usually localized to the nucleus our results may re present a specific feature of the wild-type phenotype of p53 in prosta te carcinoma cells. The localization of p53 in nucleoli may be another mechanism of the inactivation of wild-type p53.