A STUDY OF CISPLATIN-RADIOSENSITIZATION THROUGH INHIBITION OF REPAIR OF SUBLETHAL RADIATION-DAMAGE IN OVARIAN-CARCINOMA CELLS SENSITIVE ANDRESISTANT TO CISPLATIN

Human ovarian carcinoma parental cells and cisplatin resistant variant s were evaluated for the effect of cisplatin on radiosensitization and repair of sublethal radiation damage. The data showed that for 1 h ci splatin treatment (2 mu g/ml), radiosensitization was achieved only if the drug was given after irradiation. Cisplatin treatment was also ab le to inhibit repair of sublethal radiation damage (SLDR). The degree of inhibition was about the same in the resistant and the sensitive ce ll lines. In the parental line 4 mu g/ml cisplatin was required to inh ibit SLDR while in the resistant line both 2 and 4 mu g/ml produced in hibition of SLDR and in the resistant cell line the degree of SLDR inh ibition was greater for cisplatin after irradiation compared to cispla tin before irradiation.