INDUCTION OF SISTER-CHROMATID EXCHANGES BY ALUI, DNASE-I, BENZON NUCLEASE AND BLEOMYCIN IN CHINESE-HAMSTER OVARY (CHO) CELLS

Authors
OBE G SCHUNCK C JOHANNES C
Citation
G. Obe et al., INDUCTION OF SISTER-CHROMATID EXCHANGES BY ALUI, DNASE-I, BENZON NUCLEASE AND BLEOMYCIN IN CHINESE-HAMSTER OVARY (CHO) CELLS, MUTATION RESEARCH, 307(1), 1994, pp. 315-321
Citations number
29
Categorie Soggetti
Genetics & Heredity",Toxicology
Journal title
MUTATION RESEARCH → ACNP
ISSN journal
00275107
Volume
307
Issue
1
Year of publication
1994
Pages
315 - 321
Database
ISI
SICI code
0027-5107(1994)307:1<315:IOSEBA>2.0.ZU;2-I
Abstract
Various endonucleases (AluI, DNase I, benzon nuclease) and bleomycin i nduce sister-chromatid exchanges (SCE) in Chinese hamster ovary (CHO) cells. The frequencies of SCE are elevated in cells with chromosome-ty pe aberrations, only slightly elevated in cells with chromatid exchang es, and in the control range in cells without chromosomal aberrations. These data indicate that SCE are produced when DNA breaks induced in G1 are either not repaired or misrepaired.