ISOLATION AND CHARACTERIZATION OF NOVEL HETEROGENEOUS BRANCHED CYCLOMALTOOLIGOSACCHARIDES (CYCLODEXTRINS) PRODUCED BY TRANSGALACTOSYLATION WITH ALPHA-GALACTOSIDASE FROM COFFEE BEAN

Authors
KOIZUMI K TANIMOTO T OKADA Y HARA K FUJITA K HASHIMOTO H KITAHATA S
Citation
K. Koizumi et al., ISOLATION AND CHARACTERIZATION OF NOVEL HETEROGENEOUS BRANCHED CYCLOMALTOOLIGOSACCHARIDES (CYCLODEXTRINS) PRODUCED BY TRANSGALACTOSYLATION WITH ALPHA-GALACTOSIDASE FROM COFFEE BEAN, Carbohydrate research, 278(1), 1995, pp. 129-142
Citations number
11
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Carbohydrate research → ACNP
ISSN journal
00086215
Volume
278
Issue
1
Year of publication
1995
Pages
129 - 142
Database
ISI
SICI code
0008-6215(1995)278:1<129:IACONH>2.0.ZU;2-T
Abstract
Transgalactosylated derivatives of cyclomalto-hexaose (alpha CD), -hep taose (beta CD), and -octaose (gamma CD) were synthesized by cu-galact osidase from coffee bean using melibiose as a donor and alpha CD, beta CD or gamma CD as an acceptor. Mono- and di-O-alpha-D-galactosylated CDs were isolated and purified by HPLC. Their structures were elucidat ed by fast-atom bombardment mass spectrometry (FABMS) and C-13 NMR spe ctroscopy. For structural determination of positional isomers of 6(1), 6(n)-di-O-alpha-D-galactosyl-CDs, digestion products with cyclodextrin glucanotransferase were analyzed by HPLC and FABMS.