PEPTIDE ATTACHMENT TO EXTREMITIES OF LIPOSOMAL SURFACE GRAFTED PEG CHAINS - PREPARATION OF THE LONG-CIRCULATING FORM OF LAMININ PENTAPEPTIDE, YIGSR

Poly(ethylene glycol) (PEG)-grafted liposomes offer new opportunities as long-circulating platforms presenting biologically relevant ligands . In pursuit of this goal, liposomal conjugates of YIGSR were prepared by mild periodate oxidation of TYIGSR-NH2 and incubation of the produ ct with hydrazide-PEG-(distearoylphosphatidyl)ethanol amine-containing liposomes. The peptide-carrying liposomes, with up to 500 YIGSR resid ues per vesicle, despite exhibiting faster blood clearance rates than the parent liposomes in rats, remained in circulation for extended per iods of time. Mean residence times for the parent liposomal formulatio n and conjugated preparations containing 200 and 500 YIGSR residues pe r vesicle were 28, 25, and 23 h, respectively. The results have import ant implications for systemic delivery of peptides and for their use a s targeting moieties for PEG-grafted liposomes.