The growth of microorganisms in solid foods is different from that in
aqueous broths. Methods that can detect the presence of microorganisms
in situ in solids are urgently needed to further our understanding of
the presence, spatial distribution, survival and toxin production of
microorganisms in foods. As will be discussed in this article, the use
of antibody-linked probes with counter staining, coupled with cryo-se
ctioning and light microscopy, allows the sites of microbial cells and
microbial products to be detected and also provides an understanding
of the interaction of microorganisms with food components.