IMMOBILIZATION OF ENZYMES ON POLY(N-ACRYL OYLPIPERIDIN-4-ONE) - CHEMICAL INFLUENCE OF THE SUPPORT

Poly(N-acryloylpiperidin-4-one) resin is an immobilization support of enzymes as varied as urease (hydrolase), glucose oxidase (oxidoreducta se) or pronase (protease). These preparations of immobilized enzymes h ave a good storage stability, an acceptable catalytic activity and a g ood stability in continuous functioning. Moreover, the ketonic functio ns (piperidin-4-one) of the support play an important role in the stab ility of the immobilized enzyme (glucose oxidase) and even in the sele ctivity of the enzymatic catalyst (pronase). In this work, the chemica l action of the support during catalysis allows the prevention of gluc ose oxidase deactivation by hydrogen peroxide, since the hydrogen pero xide produced by glucose oxidation in the presence of oxygen is fixed on the ketonic sites by the formation of alpha-hydroxyhydroperoxide.