SELECTIVE EXTRACTION OF QUERCETRIN IN VEGETABLE DRUGS AND URINE BY OFF-LINE COUPLING OF BORONIC ACID AFFINITY-CHROMATOGRAPHY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Authors
BONGARTZ D HESSE A
Citation
D. Bongartz et A. Hesse, SELECTIVE EXTRACTION OF QUERCETRIN IN VEGETABLE DRUGS AND URINE BY OFF-LINE COUPLING OF BORONIC ACID AFFINITY-CHROMATOGRAPHY AND HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography B. Biomedical applications, 673(2), 1995, pp. 223-230
Citations number
23
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical applications
ISSN journal
15726495 → ACNP
Volume
673
Issue
2
Year of publication
1995
Pages
223 - 230
Database
ISI
SICI code
Abstract
Quercetrin, quercetin and chlorogenic acid were measured in urine or i n drugs by combination of boronic acid affinity chromatography and HPL C. Simple reversed-phase HPLC with UV detection was used to determine quercetrin in five different Solidago virgaurea drugs. For determinati on of quercetrin in human urine immobilized boronic acid was applied f or sample pretreatment. This procedure leads to a determination limit of 0.01 mu g/ml with a recovery rate of 95.3%. The first results using this method for quercetrin pharmacokinetics are presented.