ALTERNATIVE TRANSLATION INITIATION SITE USAGE RESULTS IN 2 FUNCTIONALLY DISTINCT FORMS OF THE GATA-1 TRANSCRIPTION FACTOR

Authors
CALLIGARIS R BOTTARDI S COGOI S APEZTEGUIA I SANTORO C
Citation
R. Calligaris et al., ALTERNATIVE TRANSLATION INITIATION SITE USAGE RESULTS IN 2 FUNCTIONALLY DISTINCT FORMS OF THE GATA-1 TRANSCRIPTION FACTOR, Proceedings of the National Academy of Sciences of the United Statesof America, 92(25), 1995, pp. 11598-11602
Citations number
32
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
92
Issue
25
Year of publication
1995
Pages
11598 - 11602
Database
ISI
SICI code
0027-8424(1995)92:25<11598:ATISUR>2.0.ZU;2-1
Abstract
GATA-1 is a zinc-finger transcription factor that plays a critical rol e in the normal development of hematopoietic cell lineages. In human a nd murine erythroid cells a previously undescribed 40-kDa protein is d etected with GATA-1-specific antibodies. We show that the 40-kDa GATA- 1 (GATA-1s) is produced by the use of an internal AUG initiation codon in the GATA-1 transcript. The GATA-1 proteins share identical binding activity and form heterodimers in erythroleukemic cells but differ in their transactivation potential and in their expression in developing mouse embryos.