INHIBITION OF NF-AT-DEPENDENT TRANSCRIPTION BY NF-KAPPA-B - IMPLICATIONS FOR DIFFERENTIAL GENE-EXPRESSION IN T-HELPER CELL SUBSETS

Activation of individual CD4(+) T cells results in differential lympho kine expression: interleukin 2 (IL-2) is preferentially produced by T helper type 1 (T(H)1) cells, which are involved in cell-mediated immun e responses, whereas IL-4 is synthesized by T(H)2 cells, which are ess ential for humoral immunity. The Ca2+-dependent factor NF-AT(p) plays a key role in the inducible transcription of both these lymphokine gen es, However, while IL2 expression requires the contribution of Ca2+- a nd protein kinase C-dependent signals, we report that activation of hu man IL4 transcription through the Ca2+-dependent pathway is diminished by protein kinase C stimulation in Jurkat T cells, This phenomenon is due to mutually exclusive binding of NF-AT(p) and NF-kappa B to the P sequence, an element located 69 bp upstream of the IL4 transcription initiation site, Human IL4 promoter-mediated transcription is dormregu lated in Jurkat cells stimulated with the NF-kappa B-activating cytoki ne tumor necrosis factor alpha and suppressed in RelA-overexpressing c ells, In contrast, protein kinase C stimulation or RelA overexpression does not affect the activity of a human IL4 promoter containing a mou se P sequence, which is a higher-affinity site for NF-AT(p) and a lowe r-affinity site for RelA, Thus, competition between two general transc riptional activators, RelA and NF-AT(p), mediates the inhibitory effec t of protein kinase C stimulation on IL4 expression and may contribute to differential gene expression in T-H cells.