Gb. Stark et al., CULTURED AUTOLOGOUS KERATINOCYTES SUSPENDED IN FIBRIN GLUE (KFGS) WITH ALLOGENIC OVERGRAFT FOR DEFINITIVE BURN WOUND COVERAGE, European journal of plastic surgery, 18(6), 1995, pp. 267-271
In a patient with burns of 88% TBSA autologous cultured keratinocyte f
ibrin glue suspension (KFGS) overgrafted with fresh split thickness al
logenic skin was used as definitive wound coverage after epifascial de
bridement and temporary allogenic closure. A total of 22% TBSA were cl
osed in this way including a complete leg except the foot. For compari
son, the contralateral leg was covered with conventional autologous sh
eet grafts (CEA). Histology revealed a layered squamous epithelium, an
d evidence of at least partial integration of allogenic dermis includi
ng the formation of a well-organized rete structure even after deep ne
cretomy. Besides the rapid stable closure and good skin quality, the n
ew technique has the advantages of early availability, simple handling
, easy repetition, and the transfer of actively proliferating epiderma
l cells. This is the first case proving that KFGS with allogenic overg
rafts leads to permanent epithelialization of large epifascial wounds.
The technique compared favorably with CEA with regard to availability
, handling, and stability.