DETERMINATION OF IONOPHORES IN THE TISSUES OF FOOD ANIMALS BY LIQUID-CHROMATOGRAPHY

Authors
GERHARDT GC SALISBURY CDC CAMPBELL HM
Citation
Gc. Gerhardt et al., DETERMINATION OF IONOPHORES IN THE TISSUES OF FOOD ANIMALS BY LIQUID-CHROMATOGRAPHY, Food additives and contaminants, 12(6), 1995, pp. 731-737
Citations number
NO
Categorie Soggetti
Food Science & Tenology","Chemistry Applied","Public, Environmental & Occupation Heath
Journal title
Food additives and contaminants → ACNP
ISSN journal
0265203X
Volume
12
Issue
6
Year of publication
1995
Pages
731 - 737
Database
ISI
SICI code
0265-203X(1995)12:6<731:DOIITT>2.0.ZU;2-7
Abstract
A liquid chromatographic method for determining residues of ionophores in bovine, porcine, and avian tissues is described. Tissues were extr acted with iso-octane-ethyl acetate and the extracts purified on silic a solid-phase extraction columns. Monensin, narasin and salinomycin we re detected by UV absorbance following post-column derivatization with vanillin, and lasalocid was detected underivatized using fluorescence . In muscle, kidney and far, all drugs were determined from a single s ample extract. In liver, a separate extraction was done to determine l asalocid. The detection limit for lasalocid, narasin and salinomycin w as 5 ppb; for monensin it was 2 ppb. Average recoveries were: lasaloci d-71.0%; monensin-94.3%; salinomycin-97.2%; narasin-94.1%.