CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC INVESTIGATIONS OF CYTOCHROME C(4) FROM PSEUDOMONAS-STUTZERI

Authors
KADZIOLA A LARSEN S CHRISTENSEN HM KARLSSON JJ ULSTRUP J
Citation
A. Kadziola et al., CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC INVESTIGATIONS OF CYTOCHROME C(4) FROM PSEUDOMONAS-STUTZERI, Acta crystallographica. Section D, Biological crystallography, 51, 1995, pp. 1071-1073
Citations number
14
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
51
Year of publication
1995
Part
6
Pages
1071 - 1073
Database
ISI
SICI code
0907-4449(1995)51:<1071:CAPCIO>2.0.ZU;2-P
Abstract
Cytochrome c(4) from the bacterium Pseudomonas stutzeri has been cryst allized and X-ray diffraction data have been collected to 2.2 Angstrom resolution. The crystals belong to the monoclinic system, space group P2(1) with cell parameters a = 49.49, b = 58.58, c = 63.51 Angstrom a nd beta = 96.96 degrees. The crystals contain two molecules in the asy mmetric. unit. Cytochrome c(4) is known to contain two covalently boun d haem groups per molecule and positions of the four haem Fe atoms in the asymmetric unit were determined from native anomalous-dispersion d ifferences. The shortest Fe-Fe distances found in the crystal were 15. 8, 16.9 and 19.0 Angstrom.