CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF PYRUVATE-KINASE TYPE-I FROM ESCHERICHIA-COLI

Authors
MATTEVI A VALENTINI G SPERANZA ML SARTORI P BOLOGNESI M CODA A
Citation
A. Mattevi et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF PYRUVATE-KINASE TYPE-I FROM ESCHERICHIA-COLI, Acta crystallographica. Section D, Biological crystallography, 51, 1995, pp. 1089-1091
Citations number
16
Categorie Soggetti
Crystallography,"Biochemical Research Methods",Biology
Journal title
Acta crystallographica. Section D, Biological crystallography → ACNP
ISSN journal
09074449
Volume
51
Year of publication
1995
Part
6
Pages
1089 - 1091
Database
ISI
SICI code
0907-4449(1995)51:<1089:CAPAOP>2.0.ZU;2-B
Abstract
Crystals of the fructose-1,6-bisphosphate-dependent pyruvate kinase fr om Escherichia coli have been grown using the hanging-drop vapour-diff usion technique. The space group was found to be C222(1) with cell dim ensions a = 76.8, b = 247.5, c=132.6 Angstrom. Diffraction data to 3.0 Angstrom resolution have been recorded and the enzyme molecular symme try analysed through inspection of the self-rotation function. The cry stallized protein is in the allosterically inactive T state.