ESTABLISHMENT OF IMMUNOLOGICAL PROBES TO STUDY HUMAN GONADOTROPIN-RELEASING-HORMONE RECEPTORS

Authors
KARANDE AA RAJESHWARI K SCHOL DJ HILGERS JHM
Citation
Aa. Karande et al., ESTABLISHMENT OF IMMUNOLOGICAL PROBES TO STUDY HUMAN GONADOTROPIN-RELEASING-HORMONE RECEPTORS, Molecular and cellular endocrinology, 114(1-2), 1995, pp. 51-56
Citations number
35
Categorie Soggetti
Endocrynology & Metabolism","Cell Biology
Journal title
Molecular and cellular endocrinology → ACNP
ISSN journal
03037207
Volume
114
Issue
1-2
Year of publication
1995
Pages
51 - 56
Database
ISI
SICI code
0303-7207(1995)114:1-2<51:EOIPTS>2.0.ZU;2-#
Abstract
Monoclonal antibodies were raised to a synthetic peptide corresponding to amino acids 1-29 of the human gonadotropin-releasing hormone (GnRH ) receptor. One of the two antibodies was found to recognise GnRH rece ptors on human pituitary gonadotrophs as determined by immunohistochem istry and supported by Western blotting. The antibody also bound to T4 7D human breast carcinoma cell line as determined by flow cytometric a nalysis.