Aa. Karande et al., ESTABLISHMENT OF IMMUNOLOGICAL PROBES TO STUDY HUMAN GONADOTROPIN-RELEASING-HORMONE RECEPTORS, Molecular and cellular endocrinology, 114(1-2), 1995, pp. 51-56
Monoclonal antibodies were raised to a synthetic peptide corresponding
to amino acids 1-29 of the human gonadotropin-releasing hormone (GnRH
) receptor. One of the two antibodies was found to recognise GnRH rece
ptors on human pituitary gonadotrophs as determined by immunohistochem
istry and supported by Western blotting. The antibody also bound to T4
7D human breast carcinoma cell line as determined by flow cytometric a
nalysis.