Lv. Leak et al., NITRIC-OXIDE PRODUCTION BY LYMPHATIC ENDOTHELIAL-CELLS IN-VITRO, Biochemical and biophysical research communications, 217(1), 1995, pp. 96-105
The present study demonstrates that confluent monolayer cultures of ly
mphatic endothelial cells produce and secrete NO. Immunofluorescent st
udies showed that eNOS activity can be stimulated with Ca ionophore to
enhance the production of NO. Cells and various cytokines stimulated
the production of iNOS which showed the greatest increase in activity
at 4 hrs and declined at 18 and 24 hrs. These studies provide evidence
that, within the lymphatic vascular lumen, nitric oxide may be produc
ed by the lymphatic endothelium which interact with various vasoactive
substances to regulate lymphatic vascular tone. In addition, the prod
uction of NO by LEC may be important in the regulation of lymphatic va
scular tone in order to more readily accommodate sudden fluctuations i
n lymph flow and pressure that normally occur during the process of ly
mph formation and propulsion. (C) 1995 Academic Press, Inc.