TRANSLOCATION OF INSULIN-REGULATED GLUCOSE-TRANSPORTER IS STIMULATED BY LONG-CHAIN 1,2-DIACYLGLYCEROL IN RAT ADIPOCYTES

The long-chain diacylglycerol 1,2-dimyristoylglycerol emulsified with taurodeoxycholate has been shown to potently stimulate glucose transpo rt in isolated rat adipocytes (Stralfors, Nature 335, 554-556 (1988)), We now report that this 1,a-diacylglycerol in the presence of taurode oxycholate, similarly to insulin, induced translocation of the insulin -regulated glucose transporter (GLUT-4) from a microsomal membrane com partment to the plasma membrane, H4IIE hepatoma cells expressed mRNA f or GLUT-1, but not for GLUT-4, In these, otherwise insulin-responsive, cells diacylglycerol or insulin had only a marginal effect on glucose transport. (C) 1995 Academic Press, Inc.