IMPROVED GENOMIC NUCLEAR-DNA EXTRACTION FOR 8-HYDROXYDEOXYGUANOSINE ANALYSIS OF SMALL AMOUNTS OF RAT-LIVER TISSUE

Authors
NAKAE D MIZUMOTO Y KOBAYASHI E NOGUCHI O KONISHI Y
Citation
D. Nakae et al., IMPROVED GENOMIC NUCLEAR-DNA EXTRACTION FOR 8-HYDROXYDEOXYGUANOSINE ANALYSIS OF SMALL AMOUNTS OF RAT-LIVER TISSUE, Cancer letters, 97(2), 1995, pp. 233-239
Citations number
22
Categorie Soggetti
Oncology
Journal title
Cancer letters → ACNP
ISSN journal
03043835
Volume
97
Issue
2
Year of publication
1995
Pages
233 - 239
Database
ISI
SICI code
0304-3835(1995)97:2<233:IGNEF8>2.0.ZU;2-0
Abstract
Using two different commercially available extraction kits, genomic/nu clear DNA could be recovered from rat liver samples as small as 10 mg. Background 8-hydroxydeoxyguanosine levels in such DNA were low and st able at 0.26-0.30 +/- 0.01-0.03/10(5) guanine residues. The minimum ti ssue wet weight required for the accurate 8-hydroxydeoxyguanosine anal ysis was 25 mg. The present results indicate that routine and reliable assessment of the involvement of oxidative DNA damage in the developm ent of various diseases, including cancer, is feasible using a variety of tissue sources.