THE INTERACTION BETWEEN NUCLEOPROTEINS AND THYROID RESPONSE ELEMENT (TRE) DURING REGENERATING RAT-LIVER

We examined the interaction of nucleoproteins with thyroid response el ement (TRE) by south-western method. The proteins that bound specifica lly to TRE were detected at apparent molecular weight about 30,000 and 15,000 similar to 10,000. After digestion of nucleoproteins blotted o n PVDF membrane with Endoglycosidase H, the amounts of TRE that bound to the nucleoproteins were increased. These results show that carbohyd rate moieties of nucleoproteins have an important role on the interact ion of the nucleoproteins with TRE. T-3 Stimulated the binding of TRE to nucleoproteins. The amount of T-3 binding to nucleoproteins was cha nged during liver regeneration. These results indicate that T-3 change s the interaction of the nucleoproteins with TRE, so T-3 serves as a t ransducing signal to modulate the gene regulating activity of TRE-bind ing nucleoproteins. Liver regeneration may be controlled by these modi fication of interaction of nucleoproteins with DNA.