The objective of the study was to investigate the control of female di
fferentiation by oral administration of varying doses of estradiol-17
beta (E2) in juvenile grey mullet Mugil cephalus. The concentrations o
f E2 in plasma, stomach and muscle were also measured after feeding ex
periment. Six-month-old juvenile grey mullet were divided into three g
roups, control (n = 55), low (n = 35) and high (n = 35) doses of E2, r
espectively. The low-dose diet was 1 mg E2/kg feed (period I for 4 mon
ths) and then 15 mg/kg feed (period II for another 4 months). The high
-dose diet was 8 mg E2/kg feed (period I for 4 months) and then 120 mg
E2/kg (period II for another 4 months). Low- and high- doses of E2 st
imulated development of a unisexual group of juvenile grey mullet, all
in the female direction. The effects of E2 on feminization was dose-d
ependent. E2 was able to induce the beginning of sexual differentiatio
n at a smaller body size and length in this species. E2 in muscle and
blood was not detectable in the control or low-dose groups. Higher lev
els of E2 were detected in plasma, stomach and muscle immediately afte
r the ending of treatment with high doses of E2. E2 in stomach and mus
cle in the high-dose group diminished to control levels after 45 days
of feeding with the control diet.