COMPARISON OF 6 BIOASSAYS FOR ASSESSING IN-VITRO ACUTE TOXICITY AND STRUCTURE-ACTIVITY-RELATIONSHIPS FOR VINYL-CHLORIDE MONOMER, ITS MAIN METABOLITES AND DERIVATES
Mp. Sauvant et al., COMPARISON OF 6 BIOASSAYS FOR ASSESSING IN-VITRO ACUTE TOXICITY AND STRUCTURE-ACTIVITY-RELATIONSHIPS FOR VINYL-CHLORIDE MONOMER, ITS MAIN METABOLITES AND DERIVATES, Science of the total environment, 172(1), 1995, pp. 79-92
The in vitro cytotoxicity and structure-activity-relationship (SAR) of
vinyl chloride monomer, its main metabolites (chloroethanol, chloroac
etaldehyde, acetaldehyde, chloroacetic acid) and some structurally-rel
ated substances (dichloroethane, ethanol, glycolic acid, thioglycolic
acid, ethylene glycol and di-ethylene glycol) were tested and compared
with six bioassays performed on L-929 murine fibroblasts and ciliated
protozoa Tetrahymena pyriformis GL. Major differences of cytotoxicity
exist between the tested substances. Moreover, DTP, RNA, MTT and NRI
assays appeared to be the four most sensitive assays. The best concord
ance of results was observed between MTT and NRI (r = 0.998), between
MTT and CB (r = 0.979) and between RNI and CB (r = 0.969), but also be
tween DTP and MTT (r = 0.929), between DTP and RNI (r = 0.933) and bet
ween DTP and CB (r = 0.941). Significant correlation between the in vi
tro biological responses of both models and in vivo data of tested sub
stances was noted. Cytotoxicity increased concurrently with the lipoph
ilicity coefficient; this relationship was statistically significant f
or both the in vivo and in vitro data obtained with the MTT, NRI and C
B assays.