HOLLOW-FIBER ARRAY AFFINITY-CHROMATOGRAPHY

Preparative separations of creatine phosphokinase and bovine albumin a re carried out by hollow fiber liquid chromatography. The hollow fiber s used are poly(propylene), 240 mu m in diameter. They are uniformly c oated with cross-linked poly(vinyl alcohol) and then reacted with the triazine dye Procion Blue MX-R. Experiments with these fibers show goo d protein separations, including those of the phosphokinase from the r abbit heart and the rabbit brain. Other experiments show that overload ing can be successfully predicted. In terms of resolution at low sampl e loads, hollow fiber chromatography is currently inferior to analytic al columns, though it is potentially superior for preparative separati ons.