OPTIMIZATION OF SINGLE-CELL-PROTEIN PRODUCTION FROM CASSAVA STARCH USING SCHWANNIOMYCES-CASTELLII

Schwanniomyces castellii B5285 grew faster and produced greater biomas s and higher protein yield than either S. alluvius ATCC 26074 or S, al luvius 81Y when these amylolytic yeasts were grown with 2% (w/v) cassa va starch as sole C source, With 0.5% (w/v) glutamate as N source, S, castellii reached 7.12 g cell dry mass/l, with a protein yield of 6.4 g/100 g starch, The optimal agitation speed, aeration rate and pH for growth of this yeast in a fermenter were 400 rev/min, 1.67 vol./vol.mi n, and 5.0, respectively, Tween 80 at 0.1% increased cell dry mass to 8.90 g/l, cell yield to 44 g/100 g starch and protein yield to 7.4 g/1 00 g starch.