FINDING PROSPECTIVE PARTNERS IN THE LIBRARY - THE 2-HYBRID SYSTEM ANDPHAGE DISPLAY FIND A MATCH

Authors
ALLEN JB WALBERG MW EDWARDS MC ELLEDGE SJ
Citation
Jb. Allen et al., FINDING PROSPECTIVE PARTNERS IN THE LIBRARY - THE 2-HYBRID SYSTEM ANDPHAGE DISPLAY FIND A MATCH, Trends in biochemical sciences, 20(12), 1995, pp. 511-516
Citations number
50
Categorie Soggetti
Biology
Journal title
Trends in biochemical sciences → ACNP
ISSN journal
09680004
Volume
20
Issue
12
Year of publication
1995
Pages
511 - 516
Database
ISI
SICI code
0968-0004(1995)20:12<511:FPPITL>2.0.ZU;2-U
Abstract
The two-hybrid system uses the efficacy of yeast genetic assays to ide ntify protein-protein interactions. It permits the rapid cloning of ge nes encoding products that interact with a given protein of interest. Also being developed are phage display methods that allow direct physi cal selection of binding proteins. These methods have significantly al tered strategies for analysing signaling and regulatory pathways.