MUTATIONS IN THE RET PROTOONCOGENE AND THE VON HIPPEL-LINDAU DISEASE TUMOR-SUPPRESSOR GENE IN SPORADIC AND SYNDROMIC PHEOCHROMOCYTOMAS

Phaeochromocytomas may occur sporadically, or as part of the inherited cancer syndromes multiple endocrine neoplasia (MEN) type 2, vonHippel -Lindau disease (VHL), and, rarely, in type 1 neurofibromatosis. In ME N 2, germline missense mutations have been found in one of eight codon s within exons 10, 11, 13, 14, and 16 of the RET proto-oncogene. in VH L, germline mutations within one of the three exons are responsible fo r the majority of cases. To determine if somatic mutations similar to those seen in the germline in MEN 2 or VHL disease play a role in the pathogenesis of sporadic or familial phaeochromocytomas, we analysed 4 8 sporadic tumours and tumours from 17 MEN 2 and five VHL patients for mutations in RET exons 9, 10, 11, 13, 14, 15, and 16, and the entire coding sequence of VHL. Five of 48 sporadic phaeochromocytomas had RET mutations within exons 10, 11, and 16. Of these, one was proven to be germline and two were proven to be somatic mutations. Four of 48 had VHL mutations; these included both the bilateral cases in the series ( one was proven to be a germline mutation) and two others, of which one was proven somatic.