C. Lopezmediavilla et al., CHANGES IN ADULT-RAT LIVER MITOCHONDRIAL POPULATIONS AT DIFFERENT ENERGY-STATES ANALYZED BY FLOW-CYTOMETRY, Biochimica et biophysica acta. Bioenergetics, 1232(1-2), 1995, pp. 27-32
The present work studies the changes in green fluorescence intensity a
fter Rh-123 staining of the low (LFP) and the high fluorescence popula
tions (HFP) in isolated mitochondria from rat liver. The results show
that the HFP represents a mitochondrial compartment less sensitive to
changes in energy states. In addition, it is concluded that the use of
Rh-123 to monitor changes in mitochondrial membrane potential should
be undertaken with caution because, under certain circumstances, there
is no correlation between the Rh-123 intensity of fluorescence due to
its uptake by mitochondria and previously reported changes in the mit
ochondrial membrane potential.