INFLUENCE OF CARBON AND NITROGEN-SOURCES ON GLUTATHIONE CATABOLIC ENZYMES IN CANDIDA-ALBICANS DURING DIMORPHISM

The effect of carbon sources, glucose and sucrose, and nitrogen source s such as ammonia, glutamate and L-citrulline on the activities of glu tathione metabolic enzymes has been studied, Yeast and mycelial cells were used to identify changes in activity levels of glutathione reduct ase (GSSGR), glutathione transferase (GST), glutathione peroxidase (GP X) and gamma-glutamyl transpeptidase (GGT). Enzyme activities from cel ls grown in sucrose media were lower than in glucose media regardless of the enzyme tested, morphological form, or the growth interval. In a ll enzymes except GST, activity was higher in yeast form than in mycel ia, regardless of nitrogen source, with lower activity from 24 to 72 h than at 96 h, In citrulline media, yeast form showed the maximum GST, GGT, and GPX activity. In ammonia-amended media, mycelia showed maxim um activity in GGT, whereas in glutamate media, mycelia showed the max imum activity in GST. Also, the type of nitrogen source had no effect on GPX activity in the mycelial form. Finally, changing the nitrogen s ource showed no significant effect on GSSGR activity, either in the ye ast or mycelial form.