SEPARATION OF ENANTIOMERS ON A LYSOZYME-BONDED SILICA COLUMN

Authors
HAGINAKA J MURASHIMA T SEYAMA C
Citation
J. Haginaka et al., SEPARATION OF ENANTIOMERS ON A LYSOZYME-BONDED SILICA COLUMN, Journal of chromatography, 666(1-2), 1994, pp. 203-210
Citations number
16
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography → ACNP
Volume
666
Issue
1-2
Year of publication
1994
Pages
203 - 210
Database
ISI
SICI code
Abstract
A lysozyme-bonded silica column has been developed for direct separati on of enantiomers. Lysozyme was covalently bound to porous silica mate rials using an N,N'-disuccinimidyl carbonate reaction. By using a mixt ure of phosphate buffer and organic modifier as an eluent, basic and u ncharged enantiomers were resolved, while no resolution of acidic enan tiomers was observed.