CHIRAL SEPARATIONS OF BASIC AND ACIDIC COMPOUNDS IN MODIFIED CAPILLARIES USING CYCLODEXTRIN-MODIFIED CAPILLARY ZONE ELECTROPHORESIS

Chiral separations of basic drugs and organic acids can be achieved us ing cyclodextrins or cyclodextrin derivatives as chiral selectors in s urface-modified capillaries. The influence of temperature and selector concentration on separation performance was investigated in specially modified capillaries. Separations of basic compounds at acidic pH wer e performed in capillaries modified by either dynamic or permanent coa ting by adsorption of non-ionic hydroxylic polymers. Separations of ac idic compounds could be significantly improved by use of thermally imm obilized poly(vinyl alcohol) or adsorbed cationic polymers as surface coatings. The influence of non-ionic and cationic coatings on separati ons of acidic compounds was compared with regard to resolution, separa tion efficiency and analysis time.