CHIRAL SEPARATION BY CAPILLARY ZONE ELECTROPHORESIS OF AN OPTICALLY-ACTIVE DRUG AND AMINO-ACIDS BY HOST-GUEST COMPLEXATION WITH CYCLODEXTRINS

Capillary zone electrophoresis with gamma-cyclodextrin as buffer const ituent was successfully applied to the enantiomeric separation of the neuroactive chiral drug SDZ EAA 494 s, which is a high-affinity compet itive N-methyl-D-aspartate antagonist. A borate buffer containing 20 m mol/l gamma-cyclodextrin (pH 11.0) was used (voltage 15 kV). The detec tion limit of SDZ EAA 494 s was improved by derivatization with dansyl chloride (0.1% of the antipode can be determined). The dansylation of the drug is also responsible for the enantioselectivity obtained. The electrophoretic behaviour of racemates of dansylated amino acids (Asp , Gln,Lys, Arg, Gin, His, Asn, Pro, Val, Phe) was investigated with al pha,beta,gamma-cyclodextrins at different pH values.