DIRECT RESOLUTION OF OPTICALLY-ACTIVE ISOMERS ON CHIRAL PACKINGS CONTAINING ERGOLINE SKELETONS .2. ENANTIOSEPARATION OF CARBOXYLIC-ACIDS

Structurally different carboxylic acids and dansyl derivatives of amin o acids were examined on a chiral stationary phase for high-performanc e liquid chromatography. The packing was prepared by bonding to silica gel an aminopropyl derivative of the ergot alkaloid (+)-terguride. Op timization of the enantioseparations was attained through the study of the influence of the organic modifier content and the pH and ionic st rength of the buffer in the eluent. Electrostatic and hydrophobic inte ractions between the ergot alkaloid and the analyte contribute to a la rge extent to the retention and chiral discrimination. The selector wa s shown to be effective for the resolution of dicarboxylic acids, 2-ar ylcarboxylic acids and amino acid derivatives.