STABILIZATION OF A CELL BIOCATALYST WITH BETA-GALACTOSIDASE ACTIVITY BY GLUTARALDEHYDE TREATMENT

Authors
FLORES MV VOGET CE ERTOLA RJJ
Citation
Mv. Flores et al., STABILIZATION OF A CELL BIOCATALYST WITH BETA-GALACTOSIDASE ACTIVITY BY GLUTARALDEHYDE TREATMENT, Journal of chemical technology and biotechnology, 64(4), 1995, pp. 353-360
Citations number
30
Categorie Soggetti
Engineering, Chemical",Chemistry,"Biothechnology & Applied Migrobiology
Journal title
Journal of chemical technology and biotechnology → ACNP
ISSN journal
02682575
Volume
64
Issue
4
Year of publication
1995
Pages
353 - 360
Database
ISI
SICI code
0268-2575(1995)64:4<353:SOACBW>2.0.ZU;2-A
Abstract
In the present study the stabilization with glutaraldehyde of permeabi lized, non-viable Kluyveromyces lactis cells with beta-galactosidase ( beta-D-galactoside galactohydrolase, EC 3.2.1.23) activity has been in vestigated. The influence of various factors in relation to the glutar aldehyde treatment, such as reaction time, temperature and glutaraldeh yde and cell concentration, has been considered. The cell biocatalyst showed a good storage and operational stability. The kinetic propertie s of the biocatalyst in connection with lactose hydrolysis were found to be comparable to the cell-free enzyme.