ON-COLUMN AND POSTCOLUMN DERIVATIZATION FOR CAPILLARY ELECTROPHORESISWITH LASER-INDUCED FLUORESCENCE FOR THE ANALYSIS OF SINGLE CELLS

Authors
HIETPAS PB EWING AG
Citation
Pb. Hietpas et Ag. Ewing, ON-COLUMN AND POSTCOLUMN DERIVATIZATION FOR CAPILLARY ELECTROPHORESISWITH LASER-INDUCED FLUORESCENCE FOR THE ANALYSIS OF SINGLE CELLS, Journal of liquid chromatography, 18(18-19), 1995, pp. 3557-3576
Citations number
49
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of liquid chromatography → ACNP
ISSN journal
01483919
Volume
18
Issue
18-19
Year of publication
1995
Pages
3557 - 3576
Database
ISI
SICI code
0148-3919(1995)18:18-19<3557:OAPDFC>2.0.ZU;2-7
Abstract
Derivatization methods for analysis of single cell components by capil lary electrophoresis with laser-induced fluorescence detection are rev iewed. Recent advances have lead to the use of this technique for the identification of amino acids and proteins in many different cell line s ranging in size from 8 to 140 mu m. Pre-column, on-column, and post- column methods of derivatization are examined in order to determine wh ich results in the least amount of sample handling and dilution leadin g to better detection limits for the analytes of interest.