APPLICATION OF CAPILLARY ZONE ELECTROPHORESIS FOR THE ANALYSIS OF PROTEINS, PROTEIN-SMALL MOLECULES, AND PROTEIN-DNA INTERACTIONS

Capillary electrophoresis with a polyacrylamide-coated capillary was u sed for the separation of proteins, and the study of protein-small mol ecule and protein-DNA interactions. The coating (10% T polyacrylamide) is of sufficient thickness and hydrophilicity to reduce protein adsor ption and eliminate electroosmotic flow. Data on the effect of sample solvent constituents on the peak shape and position of protein solutes was investigated. The results show that CE is a relevant and fast tec hnique for the study of protein-DNA and protein-drug interaction. Also , the addition of TRIS-buffer to the sample solution resulted in the C E focusing of some basic proteins, while others were not affected.