CAPILLARY ELECTROPHORETIC SEPARATION OF PROTEINS USING STABLE, HYDROPHILIC POLY(ACRYLOYLAMINOETHOXYETHANOL)-COATED COLUMNS

Authors
CHIARI M NESI M SANDOVAL JE PESEK JJ
Citation
M. Chiari et al., CAPILLARY ELECTROPHORETIC SEPARATION OF PROTEINS USING STABLE, HYDROPHILIC POLY(ACRYLOYLAMINOETHOXYETHANOL)-COATED COLUMNS, Journal of chromatography, 717(1-2), 1995, pp. 1-13
Citations number
34
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography → ACNP
Volume
717
Issue
1-2
Year of publication
1995
Pages
1 - 13
Database
ISI
SICI code
Abstract
Capillary electrophoretic separations of high efficiency and resolutio n were obtained using poly(acryloylaminoethoxyethanol)-coated capillar ies. The polymer was covalently attached to a silica surface previousl y modified with a alpha-methacryloxylpropyl functionality. The latter was realized by catalytic hydrosilylation of allyl methacrylate on an SiH-modified fused-silica capillary. The lifetime of the new type of c oating used at pH 8.5 was more than twice that of conventional polyacr ylamide.