APPLICATION AND OPTIMIZATION OF CAPILLARY ZONE ELECTROPHORESIS IN VITAMIN ANALYSIS

The application of capillary zone electrophoresis to the determination of ascorbic acid and biotin in vitamin mixtures (pharmaceutical formu lations and also native citrus juice and fruit beverage) was investiga ted. A method was developed for determining them together with thiamin e, nicotinamide and nicotinic acid in a single run. For ascorbic acid, addition of L-cysteine as antioxidant was necessary to obtain reliabl e results. The performance of the method was tested according to sensi tivity, detection limit and standard deviation.